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KMID : 0545120060160122000
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Journal of Microbiology and Biotechnology
2006 Volume.16 No. 12 p.2000 ~ p.2003
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Expression of Thermostable α-Glucosidase from Thermus caldophilus GK24 in Recombinant Saccharomyces cerevisiae
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Choi Jae-Youl
Ahn Jung-Oh
Kim Sun-Il
Shin Hyun-Jae
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Abstract
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A gene (GenBank AF096282) coding for a -glucosidase (TcaAG, EC 3.2.1.20) from Thermus caldophilus GK24 was expressed in Saccharomyces cerevisiae, a generally recognized as safe (GRAS) host. The thermostable -glucosidase was produced inside of the GRAS host at 0.04 unit/mg-dry cell by the constitutively expressing ADH1 promoter and at 1.2 unit/mg-dry cell by the inductively expressing GALl0 promoter, respectively. No -glucosidase activities were found in the medium when the MF-alpha signal sequence from S. cerevisiae or -amylase signal sequence from Aspergillus oryzae were fused before the -glucosidase gene for the secretion.
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KEYWORD
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recombinant enzyme, Saccharomyces cerevisiae, Thermus caldophilusGK24, α-Glucosidase, protein expression
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